Assessing the Contribution of Different Causes of Death to Life Expectancy Disparities in the United States

Life expectancy is not the same for all people in the United States. While so me enjoy life expectancies of more than 80 years, others are at risk of dying much sooner. The following studies investigate how different causes of death such as homicide, diabetes, heart disease, and drug poisoning contribute across the life span to: 1) life expectancy gaps across different sex, racial, ethnic, and education groups, and 2) life expectancy change over time for different sex, racial, ethnic, and education groups. Each study focuses on a different area of the U.S., with Chapter 2 focusing on the national-level, Chapter 3 focusing on the Great Lakes region, and Chapter 4 focusing on Washington, D.C. In Chapter 2, I find that homicide among low-educated, young males contributes to life expectancy gaps between Black and white males, and also life expectancy gaps between Hispanic and white males. Additionally, heart disease among older, higher-educated males and females contributes to life expectancy gaps between Blacks and whites. In Chapter 3, I find that drug poisoning among all Black and white males and females has contributed to reductions in life expectancy over time in the Great Lakes region, but drug poisoning has decreased life expectancy particularly for low-educated, white males and females. In Chapter 4, I find that homicide contributed most to the life expectancy gap between Black and white males at young ages in Washington, D.C., while heart disease and cancer contributed most to Black-white life expectancy gaps among both males and females at later stages of life in Washington, D.C. The findings from these studies can inform future research on life expectancy differences and guide targeted public health interventions to help reduce life expectancy disparities in the U.S

Oral History Conversation With Evan Malter

An oral history interview with Evan Malter. Evan walks through his life story, personal experiences, and how they have led to the creation of Count Loyalty, a small business that identifies loyalty capital for businesses looking to obtain a bank loan

Treadmill Exercise Improves Fracture Toughness and Indentation Modulus without Altering the Nanoscale Morphology of Collagen in Mice.

The specifics of how the nanoscale properties of collagen (e.g., the crosslinking profile) affect the mechanical integrity of bone at larger length scales is poorly understood despite growing evidence that collagen’s nanoscale properties are altered with disease. Additionally, mass independent increases in postyield displacement due to exercise suggest loading-induced improvements in bone quality associated with collagen. To test whether disease-induced reductions in bone quality driven by alterations in collagen can be rescued or prevented via exercise-mediated changes to collagen’s nanoscale morphology and mechanical properties, the effects of treadmill exercise and β-aminopropionitrile treatment were investigated. Eight week old female C57BL/6 mice were given a daily subcutaneous injection of either 164 mg/kg β-aminopropionitrile or phosphate buffered saline while experiencing either normal cage activity or 30 min of treadmill exercise for 21 consecutive days. Despite differences in D-spacing distribution (P = 0.003) and increased cortical area (tibial: P = 0.005 and femoral: P = 0.015) due to β-aminopropionitrile treatment, an overt mechanical disease state was not achieved as there were no differences in fracture toughness or 4 point bending due to β-aminopropionitrile treatment. While exercise did not alter (P = 0.058) the D-spacing distribution of collagen or prevent (P < 0.001) the β-aminopropionitrile-induced changes present in the unexercised animals, there were differential effects in the distribution of the reduced elastic modulus due to exercise between control and β-aminopropionitrile-treated animals (P < 0.001). Fracture toughness was increased (P = 0.043) as a main effect of exercise, but no significant differences due to exercise were observed using 4 point bending. Future studies should examine the potential for sex specific differences in the dose of β-aminopropionitrile required to induce mechanical effects in mice and the contributions of other nanoscale aspects of bone (e.g., the mineral–collagen interface) to elucidate the mechanism for the exercise-based improvements in fracture toughness observed here and the increased postyield deformation observed in other studies

Simultaneously sorting overlapping quantum states of light

The efficient manipulation, sorting, and measurement of optical modes and single-photon states is fundamental to classical and quantum science. Here, we realise simultaneous and efficient sorting of non-orthogonal, overlapping states of light, encoded in the transverse spatial degree of freedom. We use a specifically designed multi-plane light converter (MPLC) to sort states encoded in dimensions ranging from $d = 3$ to $d = 7$. Through the use of an auxiliary output mode, the MPLC simultaneously performs the unitary operation required for unambiguous discrimination and the basis change for the outcomes to be spatially separated. Our results lay the groundwork for optimal image identification and classification via optical networks, with potential applications ranging from self-driving cars to quantum communication systems

Identification of Telomerase RNAs from Filamentous Fungi Reveals Conservation with Vertebrates and Yeasts

Telomeres are the nucleoprotein complexes at eukaryotic chromosomal ends. Telomeric DNA is synthesized by the ribonucleoprotein telomerase, which comprises a telomerase reverse transcriptase (TERT) and a telomerase RNA (TER). TER contains a template for telomeric DNA synthesis. Filamentous fungi possess extremely short and tightly regulated telomeres. Although TERT is well conserved between most organisms, TER is highly divergent and thus difficult to identify. In order to identify the TER sequence, we used the unusually long telomeric repeat sequence of Aspergillus oryzae together with reverse-transcription-PCR and identified a transcribed sequence that contains the potential template within a region predicted to be single stranded. We report the discovery of TERs from twelve other related filamentous fungi using comparative genomic analysis. These TERs exhibited strong conservation with the vertebrate template sequence, and two of these potentially use the identical template as humans. We demonstrate the existence of important processing elements required for the maturation of yeast TERs such as an Sm site, a 59 splice site and a branch point, within the newly identified TER sequences. RNA folding programs applied to the TER sequences show the presence of secondary structures necessary for telomerase activity, such as a yeast-like template boundary, pseudoknot, and a vertebrate-like three-way junction. These telomerase RNAs identified from filamentous fungi display conserved structural elements from both yeast and vertebrate TERs. These findings not only provide insights into the structure and evolution of a complex RNA but also provide molecular tools to further study telomere dynamics in filamentous fungi